English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Cold storage of platelet concentrates (PC) has become attractive due to the reduced risk of bacterial proliferation, but in vivo circulation time of cold-stored platelets is reduced. Ca 2+  release from storage organelles and higher activity of Ca 2+  pumps at temperatures &lt; 15 °C triggers cytoskeleton changes. This is suppressed by Mg 2+  addition, avoiding a shift in Ca 2+  hemostasis and cytoskeletal alterations. We report on the impact of 2–10 mM Mg 2+  on cytoskeleton alterations of platelets from PC stored at room temperature (RT) or 4 °C in additive solution (PAS), 30% plasma. Deformation of platelets was assessed by real-time deformability cytometry (RT-DC), a method for biomechanical cell characterization. Deformation was strongly affected by storage at 4 °C and preserved by Mg 2+  addition ≥ 4 mM Mg 2+  (mean ± SD of median deformation 4 °C vs. 4 °C + 10 mM Mg 2+  0.073 ± 0.021 vs. 0.118 ± 0.023,  p  &lt; 0.01; n = 6, day 7). These results were confirmed by immunofluorescence microscopy, showing that Mg 2+   ≥ 4 mM prevents 4 °C storage induced cytoskeletal structure lesion. Standard in vitro platelet function tests showed minor differences between RT and cold-stored platelets. Hypotonic shock response was not significantly different between RT stored (56.38 ± 29.36%) and cold-stored platelets with (55.22 ± 11.16%) or without magnesium (45.65 ± 11.59%; p = 0.042, all n = 6, day 1). CD62P expression and platelet aggregation response were similar between RT and 4 °C stored platelets, with minor changes in the presence of higher Mg 2+  concentrations. In conclusion, increasing Mg 2+  up to 10 mM in PAS counteracts 4 °C storage lesions in platelets, maintains platelet cytoskeletal integrity and biomechanical properties comparable to RT stored platelets.

Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates