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Aphids such as tobacco aphid  Myzus persicae-nicotianae,  are among the most important plant viral vectors and plant viruses encode genes to interact with their vectors.  Cucumber mosaic virus  (CMV) encodes 2b protein as a suppressor of plant immune and it plays a vital role in CMV accumulation and susceptibility to aphid vectors. In this study, the resistance of tobacco plants ( Nicotiana tabacum ) to  M. p. nicotianae  was evaluated by silencing of  2b  in CMV-infected plants. However, the  pFGC-C.h  silencing gene construct was transiently expressed using  Agrobacterium tumefacience,  LBA 4404 in tobacco leaves, and four days later, the plants were mechanically inoculated by CMV (Kurdistan isolate), and then, 15 days post-inoculation 1 nonviruliferous aphid was placed on each leaf for evaluation of resistance to  M. p. nicotianae . To evaluate the tobacco plants resistance and susceptibility to  M. p. nicotianae , the number of aphids existent per tobacco leaf, life table and, demographic parameters were recorded and used as a comparison indicator. The obtained results were analyzed using the age-stage, two-sex life table. The highest number of aphids was recorded on the control CMV-infected plants, while the lowest number on CMV infected leaves expressing CMV-2b silencing construct ( pFGC-C.h ). The obtained data revealed the lowest rate for all of intrinsic rate of natural increase (r m ) (0.246/day), the rate of reproduction (r0) (17.04 females/generation), and finite rate of increase (λ) (1.279/day), on the  pFGC-C.h  treatment. The maximum generation time (T) (11.834 days) was observed on (V) treatment. However, the collected data revealed induction of resistance to tobacco aphids by silencing of CMV-2b in CMV infected plants.

Induction of resistance to Myzus persicae-nicotianae in Cucumber mosaic virus infected tobacco plants using silencing of CMV-2b gene