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Determining the viral load and infectivity of SARS-CoV-2 in macroscopic respiratory droplets, bioaerosols, and other bodily fluids and secretions is important for identifying transmission modes, assessing risks and informing public health guidelines. Here we show that viral load of SARS-CoV-2 Ribonucleic Acid (RNA) in participants’ naso-pharyngeal (NP) swabs positively correlated with RNA viral load they emitted in both droplets &gt;10    $$\upmu \hbox {m}$$  μ m and bioaerosols &lt;10    $$\upmu \hbox {m}$$  μ m directly captured during the combined expiratory activities of breathing, speaking and coughing using a standardized protocol, although the NP swabs had    $$\approx$$  ≈ 10   $$^3\times$$  3 × more RNA on average. By identifying highly-infectious individuals (maximum of 18,000 PFU/mL in NP), we retrieved higher numbers of SARS-CoV-2 RNA gene copies in bioaerosol samples (maximum of 4.8   $${\times }10^{5}$$  × 10 5 gene copies/mL and minimum cycle threshold of 26.2) relative to other studies. However, all attempts to identify infectious virus in size-segregated droplets and bioaerosols were negative by plaque assay (0 of 58). This outcome is partly attributed to the insufficient amount of viral material in each sample (as indicated by SARS-CoV-2 gene copies) or may indicate no infectious virus was present in such samples, although other possible factors are identified.

Viral load of SARS-CoV-2 in droplets and bioaerosols directly captured during breathing, speaking and coughing