English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Superoxide dismutases are important group of antioxidant metallozyme and play important role in ROS homeostasis in salinity stress. The present study reports the biochemical properties of a salt-tolerant Cu, Zn-superoxide from  Avicennia marina  ( Am _SOD).  Am _SOD was purified from the leaf and identified by mass-spectrometry. Recombinant  Am _SOD cDNA was bacterially expressed as a homodimeric protein. Enzyme kinetics revealed a high substrate affinity and specific activity of  Am _SOD as compared to many earlier reported SODs. An electronic transition in 360–400 nm spectra of  Am _SOD is indicative of Cu 2+ -binding.  Am _SOD activity was potentially inhibited by diethyldithiocarbamate and H 2 O 2 , a characteristic of Cu, Zn-SOD.  Am _SOD exhibited conformational and functional stability at high NaCl concentration as well in alkaline pH. Introgression of  Am _SOD in  E. coli  conferred tolerance to oxidative stress under highly saline condition.  Am _SOD was moderately thermostable and retained functional activity at ~ 60 °C. In-silico analyses revealed 5 solvent-accessible N-terminal residues of  Am _SOD that were less hydrophobic than those at similar positions of non-halophilic SODs. Substituting these 5 residues with non-halophilic counterparts resulted in &gt; 50% reduction in salt-tolerance of  Am _SOD. This indicates a cumulative role of these residues in maintaining low surface hydrophobicity of  Am _SOD and consequently high salt tolerance. The molecular information on antioxidant activity and salt-tolerance of  Am _SOD may have potential application in biotechnology research. To our knowledge, this is the first report on salt-tolerant SOD from mangrove.

scientific reports

Comprehensive characterization and molecular insights into the salt tolerance of a Cu, Zn-superoxide dismutase from an Indian Mangrove, Avicennia marina