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In multi-view fluorescence microscopy, each angular acquisition needs to bealigned with care to obtain an optimal volumetric reconstruction. Here,instead, we propose a neat protocol based on auto-correlation inversion, thatleads directly to the formation of inherently aligned tomographies. Our methodgenerates sharp reconstructions, with the same accuracy reachable aftersub-pixel alignment but with improved point-spread-function. The procedure canbe performed simultaneously with deconvolution further increasing thereconstruction resolution.

Beyond multi-view deconvolution for inherently-aligned fluorescence tomography