English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Our study aimed to identify the novel acaricidal compound in  Xenorhabdus szentirmaii  and  X. nematophila  using the easyPACId approach (easy Promoter Activated Compound Identification). We determined the (1) effects of cell-free supernatant (CFS) obtained from mutant strains against  T. urticae  females ,  (2) CFS of the acaricidal bioactive strain of  X. nematophila  (pCEP_kan_XNC1_1711) against different biological stages of  T. urticae,  and females of predatory mites,  Phytoseiulus persimilis  and  Neoseiulus californicus , (3) effects of the extracted acaricidal compound on different biological stages of  T. urticae,  and (4) cytotoxicity of the active substance. The results showed that xenocoumacin produced by  X. nematophila  was the bioactive acaricidal compound, whereas the acaricidal compound in  X. szentirmaii  was not determined. The CFS of  X. nematophila  (pCEP_kan_XNC1_1711) caused 100, 100, 97.3, and 98.1% mortality on larvae, protonymph, deutonymph and adult female of  T. urticae  at 7 dpa in petri dish experiments; and significantly reduced  T. urticae  population in pot experiments. However, the same CFS caused less than 36% mortality on the predatory mites at 7dpa. The mortality rates of extracted acaricidal compound (xenocoumacin) on the larva, protonymph, deutonymph and adult female of  T. urticae  were 100, 100, 97, 96% at 7 dpa. Cytotoxicity assay showed that IC 50  value of xenocoumacin extract was 17.71 μg/ml after 48 h. The data of this study showed that xenocoumacin could potentially be used as bio-acaricide in the control of  T. urticae;  however, its efficacy in field experiments and its phytotoxicity need to be assessed in future.

Relative potency of a novel acaricidal compound from Xenorhabdus, a bacterial genus mutualistically associated with entomopathogenic nematodes