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Heavy metals are naturally occurring elements that have a high atomic weight and let out in the environment by agriculture, industry, mining and therapeutic expertise and thrilling amassing of these elements pollutes the environment. In this study we have investigated the potential of garlic interplanting in promoting hyper accumulation and absorption of heavy metals to provide a basis for phytoremediation of polluted land. Monoculture and inter-plantation of garlic were conducted to investigate the absorption of cadmium and lead contamination in the land. A group of experiments with single planting (monoculture) of  Lolium perenne ,  Conyza canadensis  and  Pteris vittata  as accumulators were used. The results have shown that garlic has a potential as a hyper accumulate and absorb heavy metals. It was found that the accumulation of Cd and Pb was much higher with inter-planting. Garlic boosts up the absorption of heavy metals in  Lolium perenne  of Cd 66% and Pb 44% respectively. The Inter-planting of garlic with  Pteris vittata  promotes the Cd 26% and Pb 15%. While the maximum accumulation of Lead 87% and Cadmium 77% occurred in  Conyza canadensis  herb plant. The bacterial diversity in the soil was analyzed for each experimental soil and was found that the  Proteobacteria, Acidobacteria, Actinobacteria, Firmicutes,  and  Planctomycetes  were commonly abundant in both single planting (monoculture) of ryegrass and interplanting ryegrass with garlic habitats. Variances were observed in the bacterial floral composition of single (monoculture) and intercropping (interplant) soils. Relative abundance of bacterial taxa revealed that the proportion of  Proteobacteria, Acidobacteria , and  Actinobacteria  in the inter-planting group was slightly higher, while  Firmicutes  and  Planctomycetes  were low. This study provides the evidence to control the heavy metals contaminated soils with weed species. Growth promotion and heavy metal uptake of neighboring plants proved the specific plant-plant and plant-microbial associations with garlic plants. This inter-planting strategy can be used to improve heavy metal absorption.

Garlic (Allium sativum) based interplanting alters the heavy metals absorption and bacterial diversity in neighboring plants