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Heavy metals, including chromium, are associated with developed industrialization and technological processes, causing imbalanced ecosystems and severe health concerns. The current study is of supreme priority because there is no previous work that dealt with the modeling of the optimization of the biosorption process by the immobilized cells. The significant parameters (immobilized bacterial cells, contact time, and initial Cr 6+  concentrations), affecting Cr 6+  biosorption by immobilized  Pseudomonas alcaliphila , was verified, using the Plackett–Burman matrix. For modeling the maximization of Cr 6+  biosorption, a comparative approach was created between rotatable central composite design (RCCD) and artificial neural network (ANN) to choose the most fitted model that accurately predicts Cr 6+  removal percent by immobilized cells. Experimental data of RCCD was employed to train a feed-forward multilayered perceptron ANN algorithm. The predictive competence of the ANN model was more precise than RCCD when forecasting the best appropriate wastewater treatment. After the biosorption, a new shiny large particle on the bead surface was noticed by the scanning electron microscopy, and an additional peak of Cr 6+  was appeared by the energy dispersive X-ray analysis, confirming the role of the immobilized bacteria in the biosorption of Cr 6+  ions.

Rotatable central composite design versus artificial neural network for modeling biosorption of Cr6+ by the immobilized Pseudomonas alcaliphila NEWG-2