English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Lactobacillus reuteri  AN417 is a newly characterized probiotic strain. The activity of AN417 against oral pathogenic bacteria is unknown. We investigated the antibacterial activity of cell-free  L. reuteri  AN417 culture supernatant (LRS) against three oral pathogens:  Porphyromonas gingivalis ,  Fusobacterium nucleatum , and  Streptococcus mutans .  P. gingivalis  and  F. nucleatum  have been implicated in periodontal disease, whereas  S. mutans  causes dental caries. Exposing these oral pathogenic bacteria to LRS significantly reduced their growth rates, intracellular ATP levels, cell viability, and time-to-kill. The minimal inhibitory volume of LRS was 10% (v/v) against  P. gingivalis , 20% (v/v) for  F. nucleatum , and 30% (v/v) for  S. mutans . LRS significantly reduced the integrity of biofilms and significantly suppressed the expression of various genes involved in  P. gingivalis  biofilm formation. The  L. reuteri  AN417 genome lacked genes encoding reuterin, reuteran ,  and reutericyclin, which are major antibacterial compounds produced in  L. reuteri  strains. LRS treated with lipase and α-amylase displayed decreased antibacterial activity against oral pathogens. These data suggest that the antibacterial substances in LRS are carbohydrates and/or fatty acid metabolites. Our results demonstrate that LRS has antimicrobial activity against dental pathogenic bacteria, highlighting its potential utility for the prevention and treatment of  P. gingivalis  periodontal disease.

Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria