English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

In 2005, the Nigerian Federal Ministry of Health revised the treatment policy for uncomplicated malaria with the introduction of artemisinin-based combination therapies (ACTs). This policy change discouraged the use of Sulphadoxine-pyrimethamine (SP) as the second-line treatment of uncomplicated falciparum malaria. However, SP is used as an intermittent preventive treatment of malaria in pregnancy (IPTp) and seasonal malaria chemoprevention (SMC) in children aged 3–59 months. There have been increasing reports of SP resistance especially in the non-pregnant population in Nigeria, thus, the need to continually monitor the efficacy of SP as IPTp and SMC by estimating polymorphisms in dihydropteroate synthetase ( dhps ) and dihydrofolate reductase ( dhfr ) genes associated with SP resistance. The high resolution-melting (HRM) assay was used to investigate polymorphisms in codons 51, 59, 108 and 164 of the  dhfr  gene and codons 437, 540, 581 and 613 of the  dhps  gene. DNA was extracted from 271 dried bloodspot filter paper samples obtained from children (&lt; 5 years old) with uncomplicated malaria. The  dhfr  triple mutant I 51 R 59 N 108 ,  dhps  double mutant G 437 G 581  and quadruple  dhfr  I 51 R 59 N 108  +  dhps  G 437  mutant haplotypes were observed in 80.8%, 13.7% and 52.8% parasites, respectively. Although the quintuple  dhfr  I 51 R 59 N 108  +  dhps  G 437 E 540  and sextuple  dhfr  I 51 R 59 N 108  +  dhps  G 437 E 540 G 581  mutant haplotypes linked with  in-vivo  and  in-vitro  SP resistance were not detected, constant surveillance of these haplotypes should be done in the country to detect any change in prevalence.

scientific reports

Polymorphisms in Plasmodium falciparum dihydropteroate synthetase and dihydrofolate reductase genes in Nigerian children with uncomplicated malaria using high-resolution melting technique