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The use of plant growth promoting bacteria (PGPB) as biostimulants favors the increase of crop productivity and the improvement of yield quality. The main objective of the present study was to investigate the effect of the PGPB biostimulants ( Azotobacter chroococcum ,  Bacillus subtilis ,  Bacillus megatherium  and their mixes) and the application method (foliar and soil) on the growth, the physiology, the yield and the quality of maize. The obtained results showed that  A. chroococcum  treatment increased the chlorophyll content up to 6.1%, the photosynthetic rate up to 18.4% and the transpiration rate up to 34.3%. The highest maize yields were performed by the treatments  B. megatherium  (244.67 g) and the mix of  A. chroococcum  and  B. subtilis  (1:1) (243.67 g) when applied on the soil. The  Soil  application of the PGPB resulted in increased yield of maize from 5.5 to 13.4% compared to  control  treatment. Concerning quality characteristics,  B. subtilis  treatment increased total solids content in harvested maize seeds by 92%, as well as crude fiber content by 46% compared to control. The results confirmed that the use of PGPB could contribute as a new cultivation practice for sustainable growth, productivity and quality of grain crops.

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Effect of foliar and soil application of plant growth promoting bacteria on growth, physiology, yield and seed quality of maize under Mediterranean conditions