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Farnesyl pyrophosphate synthase (FPS) is a key enzyme that catalyzes the formation of farnesyl pyrophosphate, the main initiator for rubber chain initiation  in Hevea brasiliensis  Muell. Arg. The transcriptional regulatory mechanisms of the FPS gene still not well understood. Here, a WRKY transcription factor designated HbWRKY27 was obtained by screening the latex cDNA library applied the  HbFPS1  promoter as bait. HbWRKY27 interacted with the  HbFPS1  promoter was further identified by individual Y1H and EMSA assays. HbWRKY27 belongs to group IIe WRKY subfamily which contains a typical WRKY domain and C-X5-CX23-HXH motif. HbWRKY27 was localized to the nucleus.  HbWRKY27  predominantly accumulated in latex.  HbWRKY27  was up-regulated in latex by ethrel, salicylic acid, abscisic acid, and methyl jasmonate treatment. Transient expression of HbWRKY27 led to increasing the activity of the  HbFPS1  promoter in tobacco plant, suggesting that HbWRKY27 positively regulates the  HbFPS1  expression. Taken together, an upstream transcription factor of the key natural rubber biosynthesis gene  HbFPS1  was identified and this study will provide novel transcriptional regulatory mechanisms of the FPS gene in  Hevea brasiliensis .

HbWRKY27, a group IIe WRKY transcription factor, positively regulates HbFPS1 expression in Hevea brasiliensis