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Omega-3 polyunsaturated fatty acids (PUFAs) have been known to have beneficial effects in the prevention of various diseases. Recently, it was identified that the bioactivities of omega-3 are related to lipid mediators, called pro-resolving lipid mediators (SPMs), converted from PUFAs, so they have attracted much attention as potential pharmaceutical targets. Here, we aimed to build an efficient production system composed of enzymatic and chemical catalysis that converts docosahexaenoic acid (DHA) into lipid mediators. The cyanobacterial lipoxygenase, named Osc-LOX, was identified and characterized, and the binding poses of enzyme and substrates were predicted by ligand docking simulation. DHA was converted into three lipid mediators, a 17 S -hydroxy-DHA, a 7 S ,17 S -dihydroxy-DHA (RvD5), and a 7 S ,15 R -dihydroxy-16 S ,17 S -epoxy-DPA (new type), by an enzymatic reaction and deoxygenation. Also, two lipid mediators, 7 S ,15 R ,16 S ,17 S -tetrahydroxy-DPA (new type) and 7 S ,16 R ,17 S -trihydroxy-DHA (RvD2), were generated from 7 S ,15 R -dihydroxy-16 S ,17 S -epoxy-DPA by a chemical reaction. Our study suggests that discovering new enzymes that have not been functionally characterized would be a powerful strategy for producing various lipid mediators. Also, this combination catalysis approach including biological and chemical reactions could be an effective production system for the manufacturing lipid mediators.

Synthesis of two new lipid mediators from docosahexaenoic acid by combinatorial catalysis involving enzymatic and chemical reaction