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Tuber starch content (TSC) is a very important trait in potato  (Solanum tuberosum  L.). This study is the first to use expression quantitative trait loci (eQTL) mapping of transcript-derived markers for TSC in potato. Thirty-four differentially expressed genes were selected by comparing the RNA-seq data of contrasting bulked segregants. For the 11 candidate genes, we determined their relative expression levels across the segregating diploid potato population using RT-qPCR. We detected 36 eQTL as candidate genes distributed on all twelve potato chromosomes, and nine of them overlapped with QTL for TSC. Peaks for two eQTL, eAGPaseS-a and ePGRCRURSE5, were close to the corresponding loci of the large subunit of ADP-glucose pyrophosphorylase ( AGPaseS-a ) and the 12S globulin cruciferin gene ( PGCRURSE5 ), respectively. The eQTL peaks for  AGPaseS-a  and  PGRCRURSE5  explained 41.0 and 28.3% of the phenotypic variation at the transcript level. We showed the association of the DNA markers for  AGPaseS-a  and  PGRCRURSE5  with QTL for TSC, and significant correlation between the expression level of  PGRCRURSE5  and TSC. We did not observe a significant correlation between the expression level of  AGPaseS-a  and TSC. We concluded that the cruciferin gene  PGRCRURSE5  is a novel candidate involved in the regulation of starch content in potato tubers.

eQTL mapping of the 12S globulin cruciferin gene PGCRURSE5 as a novel candidate associated with starch content in potato tubers