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Trichosporon asahii  is a pathogenic fungus that causes deep mycosis in patients with neutropenia. Establishing an experimental animal model for quantitatively evaluating pathogenicity and developing a genetic recombination technology will help to elucidate the infection mechanism of  T. asahii  and promote the development of antifungal drugs. Here we established a silkworm infection model with a transgenic  T. asahii  strain expressing eGFP. Injecting  T. asahii  into silkworms eventually killed the silkworms. Moreover, the administration of antifungal agents, such as amphotericin B, fluconazole, and voriconazole, prolonged the survival time of silkworms infected with  T. asahii . A transgenic  T. asahii  strain expressing eGFP was obtained using a gene recombination method with  Agrobacterium tumefaciens . The  T. asahii  strain expressing eGFP showed hyphal formation in the silkworm hemolymph. Both hyphal growth and the inhibition of hyphal growth by the administration of antifungal agents were quantitatively estimated by monitoring fluorescence. Our findings suggest that a silkworm infection model using  T. asahii  expressing eGFP is useful for evaluating both the pathogenicity of  T. asahii  and the efficacy of antifungal drugs.

A novel silkworm infection model with fluorescence imaging using transgenic Trichosporon asahii expressing eGFP