English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Prokineticin receptors (PROKR1 and PROKR2) are G protein-coupled receptors which control human central and peripheral reproductive processes. Importantly, allelic variants of  PROKR2  in humans are associated with altered migration of GnRH neurons, resulting in congenital hypogonadotropic hypogonadism (CHH), a heterogeneous disease characterized by delayed/absent puberty and/or infertility. Although this association is established in humans, murine models failed to fully recapitulate the reproductive and olfactory phenotypes observed in patients harboring  PROKR2  mutations. Here, taking advantage of zebrafish model we investigated the role of  prokr1b  (ortholog of human  PROKR2 ) during early stages of GnRH neuronal migration. Real-Time PCR and whole mount  in situ  hybridization assays indicate that  prokr1b  spatial-temporal expression is consistent with  gnrh3 . Moreover, knockdown and knockout of  prokr1b  altered the correct development of GnRH3 fibers, a phenotype that is rescued by injection of  prokr1b  mRNA. These results suggest that  prokr1b  regulates the development of the GnRH3 system in zebrafish. Analysis of gonads development and mating experiments indicate that  prokr1b  is not required for fertility in zebrafish, although its loss determine changes also at the testis level. Altogether, our results support the thesis of a divergent evolution in the control of vertebrate reproduction and provide a useful  in vivo  model for deciphering the mechanisms underlying the effect of  PROKR2  allelic variants on CHH.

Prokineticin receptor 2 affects GnRH3 neuron ontogeny but not fertility in zebrafish