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This research was developed to investigate whether inoculation with  Azospirillum brasilense  in combination with silicon (Si) can enhance N use efficiency (NUE) in wheat and to evaluate and correlate nutritional and productive components and wheat grain yield. The study was carried out on a Rhodic Hapludox under a no-till system with a completely randomized block design with four replications in a 2 × 2 × 5 factorial scheme: two liming sources (with Ca and Mg silicate as the Si source and limestone); two inoculations (control - without inoculation and seed inoculation with  A .  brasilense ) and five side-dress N rates (0, 50, 100, 150 and 200 kg ha −1 ). The results of this study showed positive improvements in wheat growth production parameters, NUE and grain yield as a function of inoculation associated with N rates. Inoculation can complement and optimize N fertilization, even with high N application rates. The potential benefits of Si use were less evident; however, the use of Si can favour N absorption, even when associated with  A .  brasilense . Therefore, studies conducted under tropical conditions with Ca and Mg silicate are necessary to better understand the role of Si applied alone or in combination with growth-promoting bacteria such as  A .  brasilense .

Inoculation of Azospirillum brasilense associated with silicon as a liming source to improve nitrogen fertilization in wheat crops