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Splenic immune function was enhanced in diet-induced-obese (DIO) mice caused by  Escherichia coli . The changes in spleen function on apoptosis were still unknown. Two hundred mice in groups Lean- E. coli  and DIO- E. coli  were intranasal instillation of  E. coli . And another two hundred mice in groups Lean-PBS and DIO-PBS were given phosphate-buffered saline (PBS). Subsequently, spleen histology was analyzed. Then the rates of spleen cell (SC) apoptosis, and expression of the genes and proteins of Bcl-2, Bax, caspase-3 and caspase-9 were quantified in each group at 0 h (uninfected), 12 h, 24 h, and 72 h postinfection. The SC apoptosis rates of the DIO- E. coli  groups were lower than those of the DIO-PBS groups at 12, 24 and 72 h ( p  < 0.05). Anti-apoptotic Bcl-2 expression gene and protein of the DIO- E. coli  groups were higher than those of the DIO-PBS groups ( p  < 0.05). Gene expressions of pro-apoptotic Bax, caspase-3 and caspase-9 of the DIO- E. coli  groups were lower than those of DIO-PBS groups at 12, 24 and 72 h ( p  < 0.05). The SC apo p tosis rates of the Lean- E. coli  groups were higher than those of the Lean- PBS groups at 12 h and 24 h ( p  < 0.05). Interestingly, the SC a p optosis rates in the DIO- E. coli  groups were lower than those of the Lean- E. coli  groups at 12 h ( p  < 0.05). In conclusion, our results suggested that the DIO mice presented stronger anti-apoptotic abilities than Lean mice in non-fatal acute pneumonia induced by  E. coli  infection, which is more conducive to protecting the spleen and improving the immune defense ability of the body.

Effect of intranasal instillation of Escherichia coli on apoptosis of spleen cells in diet-induced-obese mice