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An ultrasensitive indirect competitive enzyme-linked immunosorbent assay ( ic  ELISA) using monoclonal antibodies (mAbs) was developed for the specific detection of diethylstilbestrol (DES) residues. To establish an ELISA based on mAbs, hapten diethylstilbestrol mono-carboxypropyl-ether (DES-MCPE) was chemically synthetized and then conjugated to bovine serum albumin (BSA) for immunization in mice. This  ic  ELISA was further optimized for DES determination. The sensitivity of the  ic  ELISA was found to be 0.49  μ g/kg and the limit of detection was 0.075  μ g/kg. DES residues in salmon meat and pork were tested with the recovery range from 74.0 to 85.2% and the coefficient of variation (CV) was less than 10%. Parallel analysis of DES samples from salmon meat showed comparable results from the  ic  ELISA with high-performance liquid chromatography. The  ic  ELISA provides a useful screening method for the quantitative detection of DES residues in animal-derived food.

Hapten Synthesis and the Development of an Ultrasensitive Indirect Competitive ELISA for the Determination of Diethylstilbestrol in Food Samples