English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Magnetic particle imaging (MPI) is a non-invasive, non-ionizing imagingtechnique for the visualization and quantification of magnetic nanoparticles(MNPs). The technique is especially suitable for cell imaging as it offers zerobackground contribution from the surrounding tissue, high sensitivity, and goodspatial and temporal resolutions. Previous studies have demonstrated that thedynamic magnetic behaviour of MNPs changes during cellular binding andinternalization. In this study, we demonstrate how this information is encodedin the MPI imaging signal. Through MPI imaging we are able to discriminatebetween free and cell-bound MNPs in reconstructed images. This technique wasused to image and quantify the changes that occur in-vitro when free MNPs comeinto contact with cells and undergo cellularuptake over time. The quantitativeMPI results were verified by a phenanthroline assay. The results showed a meanrelative difference of 23.8% for the quantification of cell-bound MNPs. Theinsights gained from such observations provide a new window into fundamentalbiological processes and associated pathological changes occurring at acellular level. This technique could therefore offer new opportunities for theearly diagnosis of inflammatory diseases.

3D-Imaging and Quantification of Magnetic Nanoparticle Uptake by Living Cells