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There exist differences in the heat tolerance of Chinese rose varieties, and high temperature in summer can lead to failure of blooming in non-heat-tolerant Chinese rose varieties. We cloned a heat shock protein 70 gene (designated  RcHSP70 ) from heat-tolerant varieties of Chinese rose ( Rosa hybrida  L.) to elucidate the molecular mechanism of heat tolerance and improve the quality of Chinese rose. Degenerate primers were designed for  RcHSP70  according to the 5′- and 3′-end sequences of  HSP70  genes in apple and tea.  RcHSP70  was cloned from heat-tolerant Chinese rose varieties after heat shock. The heat shock-induced expression patterns of  RcHSP70  in different Chinese rose varieties were analyzed by RT-PCR. Following heat shock (38 °C/3 h),  RcHSP70  was highly expressed in the heat-tolerant varieties but not in the non-heat-tolerant varieties, indicating a close relationship between  RcHSP70  and heat resistance in Chinese rose. To verify the function of  RcHSP70 , we constructed a prokaryotic expression recombinant vector for this gene and transformed it into  Escherichia coli  BL21. The tolerance of recombinant strains to abiotic stresses, including high temperature, low temperature, high salt, heavy metals, high pH, and oxidation, was evaluated. Additionally,  RcHSP70  was transformed into tobacco plants. Because of the overexpression of this gene, transgenic tobacco plants improved their tolerance to high temperature and cold. In addition, transgenic tobacco showed better photosynthetic performance, relative electrical conductivity and proline content than wild tobacco after heat stress and cold stress. Our findings indicate that  RcHSP70  is involved in the resistance of Chinese rose to abiotic stresses.

Expression of RcHSP70, heat shock protein 70 gene from Chinese rose, enhances host resistance to abiotic stresses