English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Coxiella burnetii , the etiologic agent of Q fever, replicates in an intracellular phagolysosome with pH between 4 and 5. The impact of this low pH environment on antimicrobial treatment is not well understood. An  in vitro  system for testing antibiotic susceptibility of  C. burnetii  in axenic media was set up to evaluate the impact of pH on  C. burnetii  growth and survival in the presence and absence of antimicrobial agents. The data show that  C. burnetii  does not grow in axenic media at pH 6.0 or higher, but the organisms remain viable. At pH of 4.75, 5.25, and 5.75 moxifloxacin, doxycycline, and rifampin are effective at preventing growth of  C. burnetii  in axenic media, with moxifloxacin and doxycycline being bacteriostatic and rifampin having bactericidal activity. The efficacy of doxycycline and moxifloxacin improved at higher pH, whereas rifampin activity was pH independent. Hydroxychloroquine is thought to inhibit growth of  C. burnetii in vivo  by raising the pH of typically acidic intracellular compartments. It had no direct bactericidal or bacteriostatic activity on  C. burnetii  in axenic media, suggesting that raising pH of acidic intracellular compartments is its primary mechanism of action  in vivo . The data suggest that doxycycline and hydroxychloroquine are primarily independent bacteriostatic agents.

The Effect of pH on Antibiotic Efficacy against Coxiella burnetii in Axenic Media