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The mitogenome of Chinese grass shrimp,  Palaemonetes sinensis , was determined through Illumina sequencing, and the basic characteristics and gene arrangement were analyzed. The mitogenome of  P .  sinensis  was 15955 bp in length, consisting of 13 protein-coding genes (PCGs), 22  tRNA  genes, 2  rRNA  genes and one control region, with tightly packed. 33 of these genes were encoded on the heavy strand, and the remainders encoded on the light strand. The composition of  P .  sinensis  mitogenome presented a strong A + T bias, which account for 66.7%. All PCGs were initiated by a canonical ATN codon, except  nad5 , which was initiated by GTG. The termination codons of the PCGs were TAA, TAG and T–. The secondary structures of 22  tRNAs  of  P .  sinensis  had the typical clover structure, except of  trnS1  owing to the lack of dihydroxyuridine (DHU) arm. Gene order comparison of  P .  sinensis  and previously-sequenced Palaemoninae revealed a unique translocation between  trnT  and  trnP  in Macrobrachium. The phylogenetic analyses showed that three Exopalaemon species formed a monophyletic group and then clustered with two Palaemon species and  P .  sinensis  successively whereas Macrobrachium clustered with  Palaemon capensis  in the other clade.

Mitochondrial genome of Chinese grass shrimp, Palaemonetes sinensis and comparison with other Palaemoninae species