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The  in vivo  functions of Atlantic salmon fatty acyl desaturases ( fads2 ),  Δ6fads2-a ,  Δ6fads2-b ,  Δ6fads2-c  and  Δ5fads2  in long chain polyunsaturated fatty acid (LC-PUFA) synthesis in salmon and fish in general remains to be elucidated. Here, we investigate  in vivo  functions and  in vivo  functional redundancy of salmon  fads2  using two CRISPR-mediated partial knockout salmon, Δ6abc/5 Mt  with mutations in  Δ6fads2-a ,  Δ6fads2-b ,  Δ6fads2-c  and  Δ5fads2 , and Δ6bc Mt  with mutations in  Δ6fads2-b  and Δ 6fads2-c . F0 fish displaying high degree of gene editing (50–100%) were fed low LC-PUFA and high LC-PUFA diets, the former containing reduced levels of eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids but higher content of linoleic (18:2n-6) and alpha-linolenic (18:3n-3) acids, and the latter containing high levels of 20:5n-3 and 22:6n-3 but reduced compositions of 18:2n-6 and 18:3n-3. The Δ6abc/5 Mt  showed reduced 22:6n-3 levels and accumulated Δ6-desaturation substrates (18:2n-6, 18:3n-3) and Δ5-desaturation substrate (20:4n-3), demonstrating impaired 22:6n-3 synthesis compared to wildtypes (WT). Δ6bc Mt  showed no effect on Δ6-desaturation compared to WT, suggesting Δ6 Fads2-a as having the predominant Δ6-desaturation activity in salmon, at least in the tissues analyzed. Both Δ6abc/5 Mt  and Δ6bc Mt  demonstrated significant accumulation of Δ8-desaturation substrates (20:2n-6, 20:3n-3) when fed low LC-PUFA diet. Additionally, Δ6abc/5 Mt  demonstrated significant upregulation of the lipogenic transcription regulator, sterol regulatory element binding protein-1 ( srebp-1 ) in liver and pyloric caeca under reduced dietary LC-PUFA. Our data suggest a combined effect of endogenous LC-PUFA synthesis and dietary LC-PUFA levels on  srebp-1  expression which ultimately affects LC-PUFA synthesis in salmon. Our data also suggest Δ8-desaturation activities for salmon Δ6 Fads2 enzymes.

CRISPR/Cas9-mediated editing of Δ5 and Δ6 desaturases impairs Δ8-desaturation and docosahexaenoic acid synthesis in Atlantic salmon (Salmo salar L.)