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The study describes a novel and environment friendly route of biosynthesis of nanohydroxyapatite (nHAP).  Bacillus licheniformis  mediated synthesis of nHAP has been carried out with different phosphate concentrations (2%, 5%, 10% and 20%  w/v ) of potassium dihydrogen orthophosphate monobasic (K 2 HPO 4 ). The synthesis is supported by a two-step mechanism – (i) solubilization of P by organic acids extracellularly secreted by the bacterial strain and (ii) gelation of P and Ca. The nHAP particles were characterized using electron microscopy and XRD analysis. Powdered crystalline particles with a size range of 30 ± 5 nm were obtained with shape and size dependent on phosphate concentrations. The particles showed no adverse effect on plant growth-promoting bacteria. Evaluation of nHAP prepared by this route with 2% P source provides scope for a wide range of applications, especially as a nano-fertilizer.

A new method for biological synthesis of agriculturally relevant nanohydroxyapatite with elucidated effects on soil bacteria