English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Tristetraprolin (TTP), encoded by the  Zfp36  gene, is a zinc-finger protein that regulates RNA stability primarily through association with 3′ untranslated regions (3′ UTRs) of target mRNAs. While TTP is expressed abundantly in the intestines, its function in intestinal epithelial cells (IECs) is unknown. Here we used a cre-lox system to remove  Zfp36  in the mouse epithelium to uncover a role for TTP in IECs and to identify target genes in these cells. While TTP was largely dispensable for establishment and maintenance of the colonic epithelium, we found an expansion of the proliferative zone and an increase in goblet cell numbers in the colon crypts of  Zfp36  ΔIEC  mice. Furthermore, through RNA-sequencing of transcripts isolated from the colons of  Zfp36  fl/fl  and  Zfp36  ΔIEC  mice, we found that expression of inducible nitric oxide synthase ( iNos  or  Nos2 ) was elevated in TTP-knockout IECs. We demonstrate that TTP interacts with AU-rich elements in the  Nos2  3′ UTR and suppresses  Nos2  expression. In comparison to control  Zfp36  fl/fl  mice,  Zfp36  ΔIEC  mice were less susceptible to dextran sodium sulfate (DSS)-induced acute colitis. Together, these results demonstrate that TTP in IECs targets  Nos2  expression and aggravates acute colitis.

Tristetraprolin targets Nos2 expression in the colonic epithelium