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Diffraction-limited focusing deep into biological tissue is challenging dueto spherical aberrations that lead to a broadening of the focal spotparticularly in axial direction. While the diffraction-limit can be restoredemploying aberration correction with a deformable mirror or spatial lightmodulator, a bulky optical setup results due to the required beam-folding. Wepropose a bi actor adaptive lens, that enables axial scanning and at the sametime correction of specimen induced spherical aberrations while offering acompact setup. Using the bi-actor lens in a confocal microscope, we showdiffraction limited axial scanning up to \SI{340}{\micro m} deep inside aphantom specimen. Applying this technique for in-vivo measurements of zebrafishembryos with reporter gene-driven fluorescence in the thyroid gland revealssubstructures of thyroid follicles.

Diffraction-limited axial scanning in thick biological tissue employing an aberration correcting adaptive lens