English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We investigated the role of the resistance-nodulation-cell division superfamily (RND) efflux system on intrinsic multidrug resistance in  Serratia marcescens . We identified eight putative RND efflux system genes in the  S .  marcescens  Db10 genome that included the previously characterized systems,  sdeXY ,  sdeAB , and  sdeCDE . Six out of the eight genes conferred multidrug resistance on KAM32, a drug hypersensitive strain of  Escherichia coli . Five out of the eight genes conferred resistance to benzalkonium, suggesting the importance of RND efflux systems in biocide resistance in  S .  marcescens . The energy-dependent efflux activities of five of the pumps were examined using a rhodamine 6 G efflux assay. When expressed in the  tolC -deficient strain of  E .  coli , KAM43, none of the genes conferred resistance on  E .  coli . When  hasF , encoding the  S .  marcescens  TolC ortholog, was expressed in KAM43, all of the genes conferred resistance on  E .  coli , suggesting that HasF is a major outer membrane protein that is used by all RND efflux systems in this organism. We constructed a  sdeXY  deletion mutant from a derivative strain of the clinically isolated multidrug-resistant  S .  marcescens  strain and found that the  sdeXY  deletion mutant was sensitive to a broad spectrum of antimicrobial agents.

Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10