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Plant cell wall degrading enzymes (PCWDEs) are the primary virulence determinants of soft rotting bacteria such as the potato pathogen,  Pectobacterium atrosepticum . The  r egulation of  s econdary  m etabolite (Rsm) system controls production of PCWDEs in response to changing nutrient conditions. This work identified a new suppressor of an  rsmB  mutation –  ECA1172  or  rsmS  (  rsm   B   s uppressor). Mutants defective in  rsmB  (encoding a small regulatory RNA), show reduced elaboration of the quorum sensing molecule ( N -3-oxohexanoyl-homoserine lactone; OHHL) and PCWDEs. However, OHHL and PCWDE production were partially restored in an  rsmB, rsmS  double mutant. Single  rsmS  mutants, overproduced PCWDEs and OHHL relative to wild type  P. atrosepticum  and exhibited hypervirulence in potato. RsmS overproduction also resulted in increased PCWDEs and OHHL. Homology searches revealed  rsmS  conservation across pathogens such as  Escherichia coli (ybaM) ,  Dickeya solani, Klebsiella pneumoniae  and  Shigella flexneri . An  rsmS  mutant of  Pectobacterium carotovorum  ATCC39048 showed bypass of  rsmB -dependent repression of PCWDEs and OHHL production.  P. carotovorum  ATCC39048 produces the β-lactam antibiotic, 1-carbapen-2-em-3-carboxylic acid (a carbapenem). Production of the antibiotic was repressed in an  rsmB  mutant but partially restored in an  rsmB, rsmS  double mutant. This work highlights the importance of RsmS, as a conserved pleiotropic regulator of virulence and antibiotic biosynthesis.

The rsmS (ybaM) mutation causes bypass suppression of the RsmAB post-transcriptional virulence regulation system in enterobacterial phytopathogens