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In this study, a novel resorufin thionocarbonate-based Hg 2+ -selective signaling probe ( RT ) for microfluidic paper-based analytical device ( μ PAD) applications is reported. The designed probe,  RT , was readily synthesized by the one-step reaction of resorufin with phenyl thionochloroformate. The  RT  probe displayed a prominent color change from yellow to pink and a marked turn-on fluorescence signaling behavior exclusively toward the Hg 2+  ion. The signaling of  RT  was due to Hg 2+ -induced hydrolysis of the phenyl thionocarbonate moiety to form the parent resorufin dye, which restored its spectroscopic properties. In addition,  RT  exhibited the Hg 2+ -selective signaling behavior without interference by coexisting environmentally relevant metal ions. The detection limit for Hg 2+  in simulated wastewater samples was estimated to be 5.8 × 10 −8  M. In particular, an  RT -equipped  μ PAD prepared using a wax printing technique enabled simple and convenient determination of Hg 2+  ions in simulated wastewater samples, with a detection limit of 5.9 × 10 −6  M.

Novel Hg2+-Selective Signaling Probe Based on Resorufin Thionocarbonate and its μPAD Application