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In the current study we compared the molecular signature of expanded mesenchymal stromal cells (MSCs) derived from selected CD271+ bone marrow mononuclear cells (CD271-MSCs) and MSCs derived from non-selected bone marrow mononuclear cells by plastic adherence (PA-MSCs). Transcriptome analysis demonstrated for the first time the upregulation of 115 and downregulation of 131 genes in CD271-MSCs. Functional enrichment analysis showed that the upregulated genes in CD271-MSCs are significantly enriched for extracellular matrix ( tenascin XB, elastin, ABI family, member 3 (NESH) binding protein, carboxypeptidase Z, laminin alpha 2  and  nephroblastoma overexpressed ) and cell adhesion ( CXCR7, GPNMB, MYBPH, SVEP1, ARHGAP6, TSPEAR, PIK3CG, ABL2  and  NCAM1 ). CD271-MSCs expressed higher gene transcript levels that are involved in early osteogenesis/chondrogenesis/adipogenesis ( ZNF145, FKBP5 ). In addition, increased transcript levels for early and late osteogenesis ( DPT, OMD, ID4, CRYAB, SORT1 ), adipogenesis ( CTNNB1, ZEB, LPL, FABP4, PDK4, ACDC ), and chondrogenesis ( CCN3/NOV, CCN4/WISP1, CCN5/WISP2 and ADAMTS-5 ) were detected. Interestingly, CD271-MSCs expressed increased levels of hematopoiesis associated genes ( CXCL12, FLT3L, IL-3, TPO, KITL ). Down-regulated genes in CD271-MSCs were associated with WNT and TGF-beta signaling, and cytokine/chemokine signaling pathways. In addition to their capacity to support hematopoiesis, these results suggest that CD271-MSCs may contain more osteo/chondro progenitors and/or feature a greater differentiation potential.

Molecular signature of human bone marrow-derived mesenchymal stromal cell subsets