English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Pseudomonas  sp. strain PTH10 can utilize  o -phthalate which is a key intermediate in the bacterial degradation of some polycyclic aromatic hydrocarbons. In this strain,  o -phthalate is degraded to 2,3-dihydroxybenzoate and further metabolized via the 2,3-dihydroxybenzoate  meta -cleavage pathway. Here, the  opa  genes which are involved in the  o -phthalate catabolism were identified. Based on the enzymatic activity of the  opa  gene products,  opaAaAbAcAd ,  opaB ,  opaC , and  opaD  were found to code for  o -phthalate 2,3-dioxygenase, dihydrodiol dehydrogenase, 2,3-dihydroxybenzoate 3,4-dioxygenase, and 3-carboxy-2-hydroxymuconate-6-semialdehyde decarboxylase, respectively. Collectively, these enzymes are thought to catalyze the conversion of  o -phthalate to 2-hydroxymuconate-6-semialdehyde. Deletion mutants of the above  opa  genes indicated that their products were required for the utilization of  o -phthalate. Transcriptional analysis showed that the  opa  genes were organized in the same transcriptional unit. Quantitative analysis of  opaAa ,  opaB ,  opaC ,  opaD ,  opaE , and  opaN  revealed that, except for  opaB  and  opaC , all other genes were transcriptionally induced during growth on  o -phthalate. The constitutive expression of  opaB  and  opaC , and the transcriptional induction of  opaD  located downstream of  opaB , suggest several possible internal promoters are existence in the  opa  cluster. Together, these results strongly suggest that the  opa  genes are involved in a novel  o -phthalate catabolic pathway in strain PTH10.

2,3-Dihydroxybenzoate meta-Cleavage Pathway is Involved in o-Phthalate Utilization in Pseudomonas sp. strain PTH10