English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Mutations in the  SALL4  gene cause human syndromes with defects in multiple organs.  Sall4  expression declines rapidly in post-gastrulation mouse embryos, and our understanding of the requirement of  Sall4  in animal development is still limited. To assess the contributions of  Sall4  expressing cells to developing mouse embryos, we monitored temporal changes of the contribution of  Sall4  lineages using a  Sall4 GFP-CreER   T2   knock-in mouse line and recombination-dependent reporter lines. By administering tamoxifen at various time points we observed that the contributions of  Sall4  lineages to the axial level were rapidly restricted from the entire body to the posterior part of the body. The contribution to forelimbs, hindlimbs, craniofacial structures and external genitalia also declined after gastrulation with different temporal dynamics. We also detected  Sall4  lineage contributions to the extra-embryonic tissues, such as the yolk sac and umbilical cord, in a temporal manner. These  Sall4  lineage contributions provide insights into potential roles of  Sall4  during mammalian embryonic development. In postnatal males, long-term lineage tracing detected  Sall4  lineage contributions to the spermatogonial stem cell pool during spermatogenesis. The  Sall4 GFP-CreER   T2   line can serve as a tool to monitor spatial-temporal contributions of  Sall4  lineages as well as to perform gene manipulations in  Sall4 -expressing lineages.

Temporal changes of Sall4 lineage contribution in developing embryos and the contribution of Sall4-lineages to postnatal germ cells in mice