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Type 2 C protein phosphatases (PP2Cs) represent the major group of protein phosphatases in plants and play important roles in various plant processes. In this study, 94  MtPP2C  genes were identified from  Medicago truncatula  and further phylogenetically classified into 13 subfamilies, as supported by exon-intron organization and conserved motif composition. Collinearity analysis indicated that segmental duplication events played a crucial role in the expansion of  MtPP2C  gene families in  M .  truncatula . Furthermore, the expression profiles of  MtPP2Cs  under different abiotic treatments were analyzed using qRT-PCR. Results showed that these  MtPP2Cs  genes displayed different expression patterns in response to drought, cold and ABA stress conditions and some of the key stress responsive  MtPP2Cs  genes have been identified. Our study presents a comprehensive overview of the  PP2C  gene family in  M .  truncatula , which will be useful for further functional characterization of MtPP2Cs in plant drought and cold stress responses.

Genome-wide Identification of PP2C Genes and Their Expression Profiling in Response to Drought and Cold Stresses in Medicago truncatula