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Essential oil derivatives are widely used for anaesthetising aquatic animals. However, the effectiveness of anaesthesia often varies according to the anaesthetic agent, species, temperature, dosage, and interactions among these factors. This study evaluated the effects of eugenol on three sizes of the shrimp  Palaemonetes sinensis  at different concentrations and temperatures. Eugenol dose, water temperature, and shrimp size were found to significantly influence anaesthesia in  P. sinensis . Induction time decreased linearly with increasing water temperature and eugenol concentration, while it increased with body weight. However, recovery times lengthened with increasing concentration and temperature, and shortened with lower body size. At 100 and 200 μL/L eugenol concentrations, the survival rates of medium and large shrimps were maintained at over 80% at all temperatures studied over 72 h recovery. However, the survival rates of small shrimps were below 60% at 24 °C and 28 °C over 5 days of recovery. These results suggest that eugenol is an effective and rapid anaesthetic for  P. sinensis , but it might have disadvantages such as slow recovery and possible mortality in small shrimps and at higher temperatures and dosages.

Anaesthetic Effects of Eugenol on Grass Shrimp (Palaemonetes sinensis) of Different Sizes at Different Concentrations and Temperatures