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Alternansucrase catalyses the sequential transfer of glucose residues from sucrose onto another sucrose molecule to form a long chain polymer, known as “alternan”. The alternansucrase-encoding gene from  Leuconostoc citreum  ABK-1 ( Lcalt ) was successfully cloned and expressed in  Escherichia coli .  Lcalt  encoded  Lc ALT of 2,057 amino acid residues; the enzyme possessed an optimum temperature and pH of 40 °C and 5.0, respectively, and its’ activity was stimulated up to 2.4-fold by the presence of Mn 2+ . Kinetic studies of  Lc ALT showed a high transglycosylation activity, with K m  32.2 ± 3.2 mM and kcat 290 ± 12 s −1 . Alternan generated by  Lc ALT ( Lc -alternan) harbours partially alternating α-1,6 and α- 1,3 glycosidic linkages confirmed by NMR spectroscopy, methylation analysis, and partial hydrolysis of  Lc -alternan products. In contrast to previously reported alternans,  Lc -alternan can undergo self-assembly, forming nanoparticles with an average size of 90 nm in solution. At concentrations above 15% (w/v),  Lc -alternan nanoparticles disassemble and form a high viscosity solution, while this polymer forms a transparent film once dried.

An α-1,6-and α-1,3-linked glucan produced by Leuconostoc citreum ABK-1 alternansucrase with nanoparticle and film-forming properties