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Here, we examined the functionality of  Lactobacillus fermentum  strain JDFM216, a newly isolated probiotic bacterium, using a  Caenorhabditis elegans  model. We determined bacterial colonization in the intestinal tract of  C. elegans  by plate counting and transmission electron microscopy and examined the survival of  C. elegans  using a solid killing assay. In addition, we employed DNA microarray analysis, quantitative real time-polymerase chain reaction, and immunoblotting assays to explore health-promoting pathways induced by probiotic bacteria in  C. elegans . Initially, we found that the probiotic bacterium  L. fermentum  strain JDFM216 was not harmful to the  C. elegans  host. Conditioning with JDFM216 led to its colonization in the nematode intestine and enhanced resistance in nematodes exposed to food-borne pathogens, including  Staphylococcus aureus  and  Escherichia coli  O157:H7. Interestingly, this probiotic strain significantly prolonged the life span of  C. elegans . Whole-transcriptome analysis and transgenic worm assays revealed that the health-promoting effects of JDFM216 were mediated by a nuclear hormone receptor (NHR) family and PMK-1 signaling. Taken together, we described a new  C. elegans -based system to screen novel probiotic activity and demonstrated that preconditioning with the probiotic  L. fermentum  strain JDFM216 may positively stimulate the longevity of the  C. elegans  host via specific pathway.

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Probiotic Lactobacillus fermentum strain JDFM216 stimulates the longevity and immune response of Caenorhabditis elegans through a nuclear hormone receptor