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Extremes of climate and hypobaric hypoxia cause poor growth performance in broiler chickens at high altitude. The present study examined the potential of  Hippophae rhamnoides  extract as phytogenic feed additive for broilers reared at 3500 m above mean sea level (MSL). Higher content of phytomolecules were recorded during characterization of the extract. Immunomodulatory activity of extract was observed in chicken lymphocytes through  in-vitro  studies. Thereafter, for  in vivo  study, 105 day old Rhode Island Red (RIR) Cross-bred chicks were randomly distributed in to control and treatments T1, T2, T3, T4, T5, and T6 which were supplemented with  H. rhamnoides  aqueous extract along with basal diet, at level of 100, 150, 200, 300, 400, and 800 mg/kg body weight of chicken, respectively. Among the experimental groups, birds in the T3 group represent the highest body weight. Furthermore, treatment group birds had shown better physio-biochemical indices as compared to control group birds. Interestingly, lower mortality rate due to ascites and coccidiosis was recorded in treatment groups and therefore, higher net return was observed. Hence, present investigation demonstrated the beneficial effect of  H. rhamnoides  extract (@200 mg/kg) at high altitude and therefore, may be used in formulation of feed additive for poultry ration.

Hippophae rhamnoides as novel phytogenic feed additive for broiler chickens at high altitude cold desert