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The front-end desaturases (Fads) are rate-limiting enzymes responsible for production of long-chain polyunsaturated fatty acids (LC-PUFA). The full spectrum of the transcriptional regulation of  fads  is still incomplete, as cloning of  fads  promoter is limited to a few species. Here, we described the cloning and characterisation of the zebrafish  fads2  promoter. Using 5′-deletion and mutation analysis on this promoter, we identified a specific region containing the sterol regulatory element (SRE) which is responsible for the activation of the  fads2  promoter. In tandem, two conserved CCAAT boxes were also present adjacent to the SRE and mutation of either of these binding sites attenuates the transcriptional activation of the  fads2  promoter. An  in vivo  analysis employing GFP reporter gene in transiently transfected zebrafish embryos showed that this 1754 bp upstream region of the  fads2  gene specifically directs GFP expression in the yolk syncytial layer (YSL) region. This indicates a role for LC-PUFA in the transport of yolk lipids through this tissue layer. In conclusion, besides identifying novel core elements for transcriptional activation in zebrafish  fads2  promoter, we also reveal a potential role for  fads2  or LC-PUFA in YSL during development.

Transcriptional activation of zebrafish fads2 promoter and its transient transgene expression in yolk syncytial layer of zebrafish embryos