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Dermatophytes are the group of filamentous fungi infecting keratinized structures such as skin, hair, and nails. Knowledge about genes and molecular mechanisms responsible for pathogenicity, as well as other biological properties of  Microsporum canis  is still relatively poor. The qRT-PCR is a reliable technique for quantifying gene expression across various biological processes, and choosing a set of suitable reference genes to normalize the expression data is a crucial step of this technique. We investigated the suitability of nine candidate reference genes:  β-act ,  β-tub ,  adp-rf ,  ef1-α ,  sdha ,  rpl2 ,  mbp1 ,  psm1 , and  rGTPa  for gene expression analysis in the dermatophyte  M. canis  in response to different carbon sources, phosphate levels, and pH shifts - factors that are extremely important and necessary for growth of dermatophyte in the host tissue. The transcription stability of these genes was evaluated using NormFinder, geNorm, BestKeeper, and RefFinder software. Regarding expression stability,  mbp1 ,  β-act , and  sdha  were the most stable housekeeping genes which we recommend for future qRT-PCR studies on  M. canis  strains. To the best of our knowledge this is the first study on selection and validation of reference genes for qRT-PCR data normalization in  M. canis  growth in culture media which promote adhesion-inducing conditions.

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Selection and validation of reference genes for qRT-PCR analysis of gene expression in Microsporum canis growing under different adhesion-inducing conditions