English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Extrinsic control of single neurons and neuronal populations is a powerful approach for understanding how neural circuits function. Adding new thermogenetic tools to existing optogenetic and other forms of intervention will increase the complexity of questions that can be addressed. A good candidate for developing new thermogenetic tools is the  Drosophila  gustatory receptor family, which has been implicated in high-temperature avoidance behavior. We examined the five members of the  Gr28b  gene cluster for temperature-dependent properties via three approaches: biophysical characterization in  Xenopus  oocytes, functional calcium imaging in  Drosophila  motor neurons, and behavioral assays in adult  Drosophila . Our results show that  Gr28bD  expression in  Xenopus  oocytes produces a non-specific cationic current that is activated by elevated temperatures. This current is non-inactivating and non-voltage dependent. When expressed in  Drosophila  motor neurons,  Gr28bD  can be used to change the firing pattern of individual cells in a temperature-dependent fashion. Finally, we show that pan-neuronal or motor neuron expression of  Gr28bD  can be used to alter fruit fly behavior with elevated temperatures. Together, these results validate the potential of the  Gr28bD  gene as a founding member of a new class of thermogenetic tools.

The Drosophila Gr28bD product is a non-specific cation channel that can be used as a novel thermogenetic tool