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Ganoderma lingzhi  ( G. lingzhi ),  G. sinense ,  G. applanatum , etc. belongs to the  Ganoderma  genus of polypore mushrooms which contain rich polysaccharides valuable for nutrition and positive medicinal effects. In order to evaluate polysaccharide content in  Ganoderma  mycelia obtained in the fermentation process quickly and accurately, in this work we employed infrared spectroscopy to examine different  Ganoderma  stains of samples from diversified sources. Through mid-infrared (mid-IR) spectroscopy, we could identify the most relevant spectral bands required for polysaccharide evaluation, and through near-infrared (NIR) spectroscopy, we could establish the quantification model for making satisfactory prediction of polysaccharide ingredient content. As such, we have achieved an effective and convenient approach to quantitative assessment of the total polysaccharides in  Ganoderma  mycelia but also demonstrated that infrared spectroscopy can be a powerful tool for quality control of  Ganoderma  polysaccharides obtained from industrial production.

Assessment of Polysaccharides from Mycelia of genus Ganoderma by Mid-Infrared and Near-Infrared Spectroscopy