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A type 2 metallothionein gene,  SsMT2 , was cloned from  Suaeda salsa , a salt- and alkali-tolerant plant, which is dominant species on the saline/alkali soil of northeast China. The  SsMT2 g ene was expressed in all organs except the flower and its expression was induced by various stresses such as CdCl 2 , NaCl, NaHCO 3 , and H 2 O 2  treatments. SsMT2-transgenic yeast ( Saccharomyces cerevisiae ) and plants ( Arabidopsis thaliana ) showed significantly increased resistance to metal, salt and oxidant stresses. These transgenics accumulated more Cd 2+ , but less Na +  than their wild type counterparts.  SsMT2  transgenic  Arabidopsis  maintained lower level of H 2 O 2  than wild type plants did in response to the stress treatments. These results demonstrated that the  SsMT2  gene plays an important role in reactive oxygen species scavenging and confers enhanced metal and oxidant tolerance to plants.

Functional characterization of a type 2 metallothionein gene, SsMT2, from alkaline-tolerant Suaeda salsa