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High resolution melting analysis (HRMA) is a single-tube method, which can be carried out rapidly as an additional step following real-time quantitative PCR (qPCR). The method enables the differentiation of genetic variation (down to single nucleotide polymorphisms) in amplified DNA fragments without sequencing. HRMA has previously been adopted to determine variability in the amplified genes of a number of organisms. However, only one work to date has focused on pathogenic parasites–nematodes from the genus  Trichinella . In this study, we employed a qPCR-HRMA assay specifically targeting two sequential gene fragments– cytochrome c oxidase subunit I  ( COI ) and  expansion segment V  ( ESV ), in order to differentiate 37 single L1 muscle larvae samples of eight  Trichinella  species. We show that qPCR-HRMA based on the mitochondrial  COI  gene allows differentiation between the sequences of PCR products of the same length. This simple, rapid and reliable method can be used to identify at the species level single larvae of eight  Trichinella  taxa.

Fast and Reliable Differentiation of Eight Trichinella Species Using a High Resolution Melting Assay