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Lactate/lactic acid is an important chemical compound for the manufacturing of bioplastics. The unicellular cyanobacterium  Synechocystis  sp. PCC 6803 can produce lactate from carbon dioxide and possesses  d -lactate dehydrogenase (Ddh). Here, we performed a biochemical analysis of the Ddh from this cyanobacterium ( Sy Ddh) using recombinant proteins.  Sy Ddh was classified into a cyanobacterial clade similar to those from Gram-negative bacteria, although it was distinct from them.  Sy Ddh can use both pyruvate and oxaloacetate as a substrate and is activated by fructose-1,6-bisphosphate and repressed by divalent cations. An amino acid substitution based on multiple sequence alignment data revealed that the glutamine at position 14 and serine at position 234 are important for the allosteric regulation by Mg 2+  and substrate specificity of  Sy Ddh, respectively. These results reveal the characteristic biochemical properties of Ddh in a unicellular cyanobacterium, which are different from those of other bacterial Ddhs.

scientific reports

Substrate Specificity and Allosteric Regulation of a d-Lactate Dehydrogenase from a Unicellular Cyanobacterium are Altered by an Amino Acid Substitution