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To improve silage quality of crop forages, bacterial inoculants are often employed. In this study,  Lactobacillus brevis  SDMCC050297 and  Lactobacillus parafarraginis  SDMCC050300 were used as inoculants to corn stover in lab silos for ensiling. At the initial stage of ensiling, the pH value of the inoculated silages reduced more drastically, and the inoculated silages had higher lactic acid and acetic acid contents. After 20 days of ensiling, a reduction in lactic acid content coupled with an increase in acetic acid and 1,2-propanediol contents was observed in inoculated silages. Furthermore, both the amount of lactic acid bacteria and the abundance of order  Lactobacillales  in inoculated silages were higher than those of controls in the whole process. Meanwhile,  Lb .  brevis  predominated before day 20 and then the dominance was shifted to  Lb .  parafarraginis  until the late stage of ensiling. In contrast, the epiphytic  Lactococcus lactic  and  Lb .  plantarum  played major roles at the beginning of naturally fermented silages and then  Lb .  plantarum  and  Lb .  brevis  were the most abundant at the later stage. In conclusion, these two selected strains had capability of improving the silage quality and providing the reproducible ensiling process, thus having the potential as silage inoculants.

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Effects of inoculants Lactobacillus brevis and Lactobacillus parafarraginis on the fermentation characteristics and microbial communities of corn stover silage