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Studies on the halotolerance of bacteria are attractive to the fermentation industry. However, a lack of sufficient genomic information has precluded an investigation of the halotolerance of  Halomonas beimenensis . Here, we describe the molecular mechanisms of saline adaptation in  H. beimenensis  based on high-throughput omics and Tn5 transposon mutagenesis. The  H. beimenensis  genome is 4.05 Mbp and contains 3,807 genes, which were sequenced using short and long reads obtained via deep sequencing. Sixteen Tn5 mutants with a loss of halotolerance were identified. Orthologs of the mutated genes, such as  nqrA ,  trkA ,  atpC ,  nadA , and  gdhB , have significant biological functions in sodium efflux, potassium uptake, hydrogen ion transport for energy conversion, and compatible solute synthesis, which are known to control halotolerance. Other genes, such as  spoT ,  prkA ,  mtnN ,  rsbV ,  lon ,  smpB ,  rfbC ,  rfbP ,  tatB ,  acrR1 , and  lacA , function in cellular signaling, quorum sensing, transcription/translation, and cell motility also shown critical functions for promoting a halotolerance. In addition, KCl application increased halotolerance and potassium-dependent cell motility in a high-salinity environment. Our results demonstrated that a combination of omics and mutagenesis could be used to facilitate the mechanistic exploitation of saline adaptation in  H. beimenensis , which can be applied for biotechnological purposes.

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Revealing the Saline Adaptation Strategies of the Halophilic Bacterium Halomonas beimenensis through High-throughput Omics and Transposon Mutagenesis Approaches