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Protein derived adhesives are extensively explored as a replacement for synthetic ones, but suffers from weak adhesion and water resistance. Graphite oxide (GO) has been extensively used in nanocomposites, but not in adhesives applications. The objectives of this study were to prepare functionally improved protein adhesive by exfoliating GO with different oxidation levels, and to determine the effect of GO on adhesion mechanism. GO were prepared by oxidizing graphite for 0.5, 2, and 4 h (GO-A, GO-B and GO-C, respectively). Increasing oxidation time decreased C/O ratio; while the relative proportion of C-OH, and C = O groups initially increased up to 2 h of oxidation, but reduced upon further oxidation. Canola protein-GO hybrid adhesive (CPA-GO) was prepared by exfoliating GO at a level of 1% (w/w). GO significantly increased ( p  <  0.05 ) adhesion; where GO-B addition showed the highest dry, and wet strength of 11.67 ± 1.00, and 4.85 ± 0.61 MPa, respectively. The improvements in adhesion was due to the improved exfoliation of GO, improved adhesive and cohesive interactions, increased hydrogen bonding, increased hydrophobic interactions and thermal stability of CPA-GO. GO, as we proposed for the first time is easier to process and cost-effective in preparing protein-based adhesives with significantly improved functionalities.

Graphite Oxide Improves Adhesion and Water Resistance of Canola Protein–Graphite Oxide Hybrid Adhesive