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The aminopeptidase gene from  Mesorhizobium  SEMIA3007 was cloned and overexpressed in  Escherichia coli . The enzyme called MesoAmp exhibited optimum activity at pH 8.5 and 45 °C and was strongly activated by Co 2+  and Mn 2+ . Under these reaction conditions, the enzyme displayed K m  and k cat  values of 0.2364 ± 0.018 mM and 712.1 ± 88.12 s −1 , respectively. Additionally, the enzyme showed remarkable stability in organic solvents and was active at high concentrations of NaCl, suggesting that the enzyme might be suitable for use in biotechnology. MesoAmp is responsible for 40% of the organism’s aminopeptidase activity. However, the enzyme’s absence does not affect bacterial growth in synthetic broth, although it interfered with biofilm synthesis and osmoregulation. To the best of our knowledge, this report describes the first detailed characterization of aminopeptidase from  Mesorhizobium  and suggests its importance in biofilm formation and osmotic stress tolerance. In summary, this work lays the foundation for potential biotechnological applications and/or the development of environmentally friendly technologies and describes the first solvent- and halo-tolerant aminopeptidases identified from the  Mesorhizobium  genus and its importance in bacterial metabolism.

Halotolerant aminopeptidase M29 from Mesorhizobium SEMIA 3007 with biotechnological potential and its impact on biofilm synthesis