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The yeast Sup35 protein is a subunit of the translation termination factor, and its conversion to the [ PSI   + ] prion state leads to more translational read-through. Although extensive studies have been done on [ PSI   + ], changes at the proteomic level have not been performed exhaustively. We therefore used a SILAC-based quantitative mass spectrometry approach and identified 4187 proteins from both [ psi   − ] and [ PSI   + ] strains. Surprisingly, there was very little difference between the two proteomes under standard growth conditions. We found however that several [ PSI   + ] strains harbored an additional chromosome, such as chromosome I. Albeit, we found no evidence to support that [ PSI   + ] induces chromosomal instability (CIN). Instead we hypothesized that the selective pressure applied during the establishment of [ PSI   + ]-containing strains could lead to a supernumerary chromosome due to the presence of the  ade1-14  selective marker for translational read-through. We therefore verified that there was no prevalence of disomy among newly generated [ PSI   + ] strains in absence of strong selection pressure. We also noticed that low amounts of adenine in media could lead to higher levels of mitochondrial DNA in [ PSI   + ] in  ade1-14  cells. Our study has important significance for the establishment and manipulation of yeast strains with the Sup35 prion.

The [PSI +] yeast prion does not wildly affect proteome composition whereas selective pressure exerted on [PSI +] cells can promote aneuploidy